Main

1. Scientific activity
2. Main scientific results

Doctor of Science in Organic Chemistry (Dr.Sci.) Associate Professor in Organic Chemistry Chair, Chemistry Faculty, National Taras Shevchenko University of Kyiv, Volodymyrska 64, 01033 Kyiv, Ukraine, a Member of European Photochemistry Association (EPA), a Member of American Chemical Society, e-mail address: pvg@univ.kiev.ua.

He head a group of students and Ph.D. students in the works on design and synthesis of multichannel fluorescent probes and ion indicators on the basis of flavonols, 3-hydroxyquinolones, dicyclopentane[b,e]pyridines and ketocyanines. The group collaborates with many scientific groups in the world, for example from France (Faculte de Pharmacie, Universite Louis Pasteur, Strasbourg, Faculte de Chimie, Paul Sabatier University, Toulouse,), USA (University of Kansas Medical Center, Kansas City, KS, Institute of Molecular Biophysics and Department of Chemistry, Florida State University, Tallahassee, FL), Poland (Gdansk University, Gdansk), Sweden (Department of Medical Cell Biology Biomedical Centrum, Uppsala University), Turkey (Institute of Biology, TUBITAK Marmara Research Center), Belarus (Institute of Physics, BAS, Minsk) the Netherlands (Vrije Universiteit, Department of Analytical Chemistry and Applied Spectroscopy, Amsterdam) and Spain (Departamento de Quimica Fisica Aplicada, Universidad Autonoma de Madrid). The most intensive collaboration takes place with the scientists from Institute for Chemistry at Kharkiv National University and from A.V. Paladin Institute of Biochemistry, NAS, Kyiv, Ukraine. Joint results are published in the top scientific journals. The group took place in the European scientific project INTAS (grant 96-1225: "Crowned ketocyanine dyes as a new generation of fluorescent probes sensitive to biomembrane surface potential"), French-Ukrainian programs of scientific collaboration "DNIPRO" and "EcoNet", as well as "Visby" program of international scientific collaboration of Sweden.

• A series of multiparametric fluorescence probes on the basis of 3-hydroxyflavones were developed. Due to increased volume of transmitted information the probes allow to measure separately polarity and hydration (H-bond donating ability) of a medium.
• First probe for the detection of adenosine triphosphate (ATP) with high selectivity to this analyte was created.
• Wide range fluorescent pH indicator on the basis of flavonol was elaborated.
• Several probes for the estimation of lipid membrane state and dipole potential were elaborated on the basis of 3-hydroxyflavones.
• Diflavonols - a new class of multiparametric fluorescent probes that utilize excited state intramolecular proton transfer effect was designed and synthesized for study of characteristics of liquid phases.
• Dicyclopenta[b,e]pyridines, a new class of fluorescent dyes with emission in a whole range of visible light was created and studied. A series of fluorescent pH indicators based on generation of ratiometric signal were studied.
• Several crowned ketocyanine and crowned flavonol sensors have been designed and synthesized. Sensors displayed record band separation in the fluorescence spectra at binding with Mg²⁺, Ba²⁺ and H⁺ cations in a result of complex formation by different binding sites of the molecule.
• The nature of ketocyanine complexes with protic molecules at their different concentrations in aprotic medium was estimated, that gives an opportunity to evaluate hydration level of various layers of lipid membranes, including biomembranes.